Fig. 1 | Scientific Reports

Fig. 1

From: NPS-2143 suppresses malignant phenotypes of retinoblastoma cells involved in regulating NF-κB pathway

Fig. 1

NPS-2143 inhibited cell proliferation and promoted cell apoptosis of human retinoblastoma cell line. (A) Expression of CaSR was detected in ARPE-19 and Y-79 cells. (B) Concentration of NPS-2143 treatment was determined by drug sensitivity assay and 16.5 µM was chosen as IC50 value of NPS-2143 treatment. (C) Expression of CaSR was detected in Y-79 cells after NPS-2143 treatment. (D) The proliferation of Y-79 cells was examined by CCK8 assay. Compared with control group, the cell proliferation of NPS-2143 treated group decreased and showed significant difference at 48 h and 72 h in Y-79 cell lines. E-F. Apoptotic cell numbers of control and NPS-2143 treated group were examined by flow cytometry, the apoptotic cells level in NPS-2143 treated group was significantly higher than that in control group. G. Western blotting was used to examine the expression of apoptosis related genes in control and NPS-2143 treated group. As a result, compared with control group, the expression of Caspase3 and BAX were up-regulated and Bcl-2 down-regulated significantly in Y-79 cells treated by NPS-2143. **P < 0.05 vs. control.

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