Fig. 1
VSVd51 over-express miR-199 in TNBC cell lines. (a) Graphical representation of VSVd51 expressing pri-miR-199a-5p or pre-miR-199a-5p. The transgene is inserted between VSV-G and VSV-L. To design VSVd51-pri-miR-199, the sequence of MIR199 A1 was flanked by 50 nucleotides (nts) corresponding to the genomic DNA (gDNA) sequence upstream and downstream of the MIR199 A1 gene. (b) Cellular viability (MTT assay) of 4 T1 cells following infections with 10−5 to 101 plaque forming units per cells (MOI). (c) Quantification of relative miR-199 expression (stem-loop qPCR) in 4 T1 cells infected with VSVd51-pri-miR-199 (V1) or VSVd51-pre-miR-199 (V2) presented as fold increase. (d) Cellular viability (MTT assay) of MDA-MB-231 following infections with 10−5 to 101 plaque forming units per cells (MOI). (e) Quantification of relative miR-199 expression (stem-loop qPCR) in MDA-MB-231 cells infected with VSVd51-pri-miR-199 (V1) or VSVd51-pre-miR-199 (V2) presented as fold increase.
