Fig. 5 | Scientific Reports

Fig. 5

From: Therapeutic limitations of oncolytic VSVd51-mediated miR-199a-5p delivery in triple negative breast cancer models

Fig. 5

Zeb1 mRNA, but not protein expression is significantly reduced by VSVd51-miR-199 in the human MDA-MB-231 TNBC cell line. (a) Quantification of ZEB1, GRP78, and MLK3 relative protein expression (Western Blot) in MDA-MB-231. (b) Representative Western blot (cropped, full blot images in Supplementary Fig. S2) of whole-cell protein extract from non-infected control MDA-MB-231 (1) and infected with VSVd51-shNTC (2), VSVD51-pri-miR-199 (3), and VSVd51-pre-miR-199 (4) (c) Relative mRNA quantification (qPCR) of ZEB1 in MDA-MB-231. One-way ANOVA with Tukey’s multiple comparison test (ns p > 0.05; * p ≤ 0.05).

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