Fig. 4
A stable cell line overexpressing human Trx-1 was successfully constructed. (A) The structure diagram of LV-Trx-1. (B) HT22 cells were transfected with LV-Vector and LV-Trx-1 and then screened with puromycin (2 µg/ml). HT22 cells stably overexpressing human Trx-1 were obtained. (C) RNA agarose gel electrophoresis for RNA extraction. (D) The mRNA expression of human Trx-1 was measured by qRT-PCR. (E) The protein expression levels of human Trx-1 and mouse Trx-1 were assessed by WB. (F) Gray scale analysis of human Trx-1 and mouse Trx-1 expression in HT22 cells overexpressing Trx-1 or treated with lentivirus alone; The quantitative analysis was based on three independent experiments. Statistics were calculated using one-way ANOVA with Tukey’s multiple comparisons test and are presented as mean ± SD from three independent experiments. ***P < 0.001.
