Fig. 5
Trx-1 overexpression in HT22 cells resisted the neurotoxicity of Aβ25−35. (A) Cell morphology of HT22 cells overexpressing human Trx-1 treated for 48 h with Aβ25−35 (40 µM). (B) Cell viability was measured by MTT analysis. Trx-1 overexpression reversed the viability of HT22 cells. (C) The increase in the extracellular LDH content was detected with LDH Cytotoxicity Assay Kit. Trx-1 overexpression inhibited the release of LDH. (D) Overexpression of Trx-1 inhibited Aβ25−35-induced apoptosis in HT22 cells (red fluorescence). Annexin V-mCherry can be coupled to phosphatidylserine on the outer membrane of apoptotic cells, and mCherry labeled with red fluorescent protein can indicate apoptotic cells. (E) Statistical analysis of Aβ25−35-induced apoptosis in HT22 cells. Red fluorescence intensity was quantified by using ImageJ. (F) Overexpression of Trx-1 inhibited the Aβ25−35-induced caspase-3 activation in HT22 cells. (G) Gray scale analysis of caspase-3 expression in HT22 cells; The quantitative analysis was based on three independent experiments. Statistics were calculated using one-way ANOVA with Tukey’s multiple comparisons test and are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.
