Fig. 6
Trx-1 inhibited the activation of NLRP3 by Aβ25−35 in HT22 cells. (A) The WB detection of NLRP3, caspase-1, GSDMD and IL-1β in WT HT22 and Trx-1 overexpressing HT22 cells treated by Aβ25−35 (40 µM). (B) Gray scale analysis of NLRP3. (C) Gray scale analysis of caspase-1. (D) Gray scale analysis of GSDMD. (E) Gray scale analysis of IL-1β. (F) Immunofluorescence staining of NLRP3 in WT HT22 and Trx-1 overexpressing HT22 cells treated by Aβ25−35 (40 µM). (G) Transfection with Trx-1 siRNA for 24 h downregulated the protein levels of Trx-1 in HT22 cells. (H) Trx-1 knockdown in HT22 cells by siRNA transfection further aggravated the activation of NLRP3. (I) The increase of NLRP3 by Aβ25−35 was abolished in SH-SY5Y cells treated with rhTrx-1 (50 µg/ml). Statistics were calculated using one-way ANOVA with Tukey’s multiple comparisons test and are presented as mean ± SD from three independent experiments. The quantitative analysis was based on three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.
