Table 1 PCR cycling conditions.

From: Thioredoxin-1 inhibits NLRP3-mediated pyroptosis by regulating TXNIP in models of Alzheimer’s disease

Steps

Temperature

Time

Cycles

Initial Denaturation

95 °C

5 min

1

Denaturation

95 °C

30 s

27-35cycles

Annealing

55 °C ~ 72 °C

30 s

Extension

72 °C

30 ~ 60 s/kb

Final extension

72 °C

10 min

1

Hold

12 °C

0

1

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