Fig. 6
From: Image guided construction of a common coordinate framework for spatial transcriptome data
Coordinate consolidation of real spatial transcriptome slices from a human dorsolateral prefrontal cortex (DLPFC). (a): Four serial dissections of the dorsolateral prefrontal cortex. (b): Superimposed spatial coordinates of the QC-validated tissue spots from four DLPFC40 slices before and after alignment by different methods, color-coded by their tissue domain annotations in the original publication. (c-d): Quantitative evaluation of tissue domain consistency across the four slices before and after alignment, using Spatial Coherence Score (c) and Mean Pairwise Adjusted Rand Index (d). STUtility does not offer de novo alignments so their values are averaged over four alignments, each using a different slice as the fixed template, with error bars marking the standard errors. (e): Spatial patterns of representative genes before and after alignment by STaCker and other programs. The displayed expression values are the natural logarithm transformation of the normalized UMI count (104 total UMI counts per spot). (f): Comparison of Moran’s I spatial autocorrelations of representative genes before and after alignment by various programs.
