Fig. 7
From: Image guided construction of a common coordinate framework for spatial transcriptome data
Coordinate consolidation of real spatial transcriptome slices from independent replicates of mouse olfactory bulbs. (a): Four biological replicates of dissected mouse olfactory bulbs. (b): Superimposed spatial coordinates of the QC-validated tissue spots from four slices before and after alignment by different methods, color-coded by the tissue domain annotations derived upon the transcriptome of the spots. (c-d): Quantitative evaluation of tissue domain consistency across the four slices before and after alignment, using Spatial Coherence Score (c) and Mean Pairwise Adjusted Rand Index (d). STUtility does not offer de novo alignments so their values are averaged over four alignments, each using a different slice as the fixed template. The standard errors are shown as error bars. (e): Spatial patterns of representative genes before and after alignment by STaCker and the other programs. The displayed expression is after the natural logarithm transformation of the normalized UMI count (104 total UMI counts per spot). (f): Comparison of Moran’s I spatial autocorrelations of representative genes before and after alignment by various programs.
