Table 2 Primer sequences for target gene annealing.
From: miRNA-200a suppresses GNAI1 and PLCB4 to modulate skin pigmentation in cashmere goats
Gene | Primer Sequence (5′→3′) |
|---|---|
GNAI1-WT-F | TCGAGTGTGAAACTGAAGGACAGTGTTAAAGCTGGGCTCTAGTGC |
GNAI1-WT-R | GGCCGCACTAGAGCCCAGCTTTAACACTGTCCTTCAGTTTCACAC |
PLCB4-WT-F | TCGAGCAAAACAATTTGCTACAGTGTTAATCTGCATGGTCTTTGC |
PLCB4-WT-R | GGCCGCAAAGACCATGCAGATTAACACTGTAGCAAATTGTTTTGC |
GNAI1-MT-F | TCGAGTGTGAAACTGAAGGACCGTATTAAAGCTGGGCTCTAGTGC |
GNAI1 -MT-R | GGCCGCACTAGAGCCCAGCTTTAATACGGTCCTTCAGTTTCACAC |
PLCB4-MT-F | TCGAGCAAAACAATTTGCTACAGCGTCAATCTGCATGGTCTTGC |
PLCB4-MT-R | GGCCGCAAAGACCATGCAGATTAACACTGTAGCAAATTGTTTTGC |
