Fig. 2
Metabolite pool sizes are driven by individual GBM rather than standard or ketogenic media. (a) Heatmap displaying pool sizes in CA3 and CA7 in both media conditions. (b) Principal components analysis (PCA) plot with minimal separation based on media, but strong separation by cell line. (c) Interaction effect examining Cell Line and Media Treatment based on two-way ANOVA that shows differences in pool sizes are based entirely on GBM cell line rather than media group. (a-c) were generated using Metaboanalyst software. (d–f) Pool size quantification of individual metabolites in CA3 and CA7 GBM cells after exposure to standard or ketogenic media. A significant decrease was found in CA3 cells in (d) lactate, (h) fumarate, (i) malate, and (l) glutamine after the ketogenic media compared to the standard media. Data are represented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by two-way ANOVA.
