Fig. 5 | Scientific Reports

Fig. 5

From: Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies

Fig. 5

VHH2SMT3 can be conjugated to a solid support for retrieval of Smt3p-tagged proteins from bacterial extracts. (A) Workflow showing purification done using a Ni–NTA agarose column and VHH2SMT3 conjugated Sepharose beads. (B) SDS PAGE gel showing different stages of purification of His6-Smt3p-Ube2G2 (top) and Smt3p-GFP-SBP-His6 (bottom). Lys: Lysate, FT: Flow through, WT: Wash through, El: Elution.

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