Table 1 Details of primers used in the study for the detection of Spodoptera Frugiperda.
Sl. No. | Assays | Primer | Primer sequences (5’→ 3’) | Annealing temperature |
|---|---|---|---|---|
1. | PCR | Forward | TGAAGCTATCCGTCTGGTTG | 52 ºC |
Reverse | TGACGTCAGCAATCTTGACA | |||
2. | LAMP | F3 | TGTCTGATTTTGCGTTGC | 60 ºC |
B3 | ACCTATAAACACTGGTTGGAAT | |||
FIP | ACTACCTAGCGGGTTTACCGTTTTTAAAAAAAAAAAAACACCCCGTTC | |||
BIP | GCAGCTCTGGGTGTGTTCATTTTTTTGTTTTTTTGGGATACGCATTA | |||
LF | TCTGGCTTGATAAGCAGGAGTAG | |||
LB | CCCCGGTAAACCCGCTAGGT | |||
3. | RPA | Forward | TCCAACCATTGCAAGACCGAACTATTTTGAAAAC | 39 ºC |
Reverse | TCCCTTTTTGACGGAGGTCGTGGAAACAACGGCTG |
