Fig. 2

Influence of Gpr109A and Niacin Treatment on Protein Expression in Primary Macrophages Co-cultured with H22 Cells. (A) Protein expression analysis of PKA, p-PPARγ, MerTK, p-STAT3 and p-STAT6 in Gpr109A WT and Gpr109A^−/− primary macrophages with or without Niacin treatment; (B) Comparison of the expression levels of IL-10, TGF-β, Cathepsin K, Cathepsin S, CD163, CD206, CCL18, and PD-L1 in Gpr109A WT and Gpr109A^−/− primary macrophages with or without Niacin treatment; (C) Comparison of the expression levels of PKA, p-PPARγ, MerTK, and IL-10 in primary macrophages from the Gpr109A WT DMSO group, Gpr109A^−/− DMSO group, Gpr109A WT-NC DMSO group, and Gpr109A-OE DMSO group; GAPDH as control protein; Data are expressed as mean ± standard deviation; N = 3; **P < 0.01; *P < 0.05.