Fig. 3 | Scientific Reports

Fig. 3

From: Glutathione-associated redox regulation alleviates radio-resistance of canine cancer stem-like cells with low proteasome activity

Fig. 3

ROS accumulation after X-irradiation and GSH contents in ZsG+ cells. (A) Flow cytometric analysis of ROS accumulation in ZsG− and ZsG+ cells after 0–5 Gy of X-irradiation. (B) Comparative analysis using the results of ROS accumulation normalized to the CellROX fluorescence of ZsG− cells in HMPOS–ZsG without X-irradiation. (C) Relative GSH contents of ZsG− and ZsG+ cells at 6–12 h of incubation after cell sorting were determined in HMPOS–ZsG and MegTCC–ZsG. Error bars correspond to the standard deviation of the mean of three independent experiments. Gray circles indicate the value from an individual biological replicate. Tukey’s multiple comparison test was used for statistical analysis. *p < 0.05, **p < 0.01, and ***p < 0.001. ROS, reactive oxygen species; GSH, glutathione.

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