Fig. 2

A CTLA-4 deficiency drives a less severe and long lasting disease. C57BL/6 J (WT), Cd4^Cre/ERT+; Ctla4^fl/fl, and Cd4^Cre/ERT−; Ctla4^fl/fl mice were given tamoxifen one week before EAU immunization and monitored by slit lamp examination until resolution. The mean ± SEM EAU scores of mice at the indicated time point of EAU induction is shown for WT (n = 15) with Cd4^Cre/ERT−; Ctla4^fl/fl (n = 8, Cre-, A) or Cd4^Cre/ERT+; Ctla4^fl/fl (n = 10, Cre+, B). The images under the graph correspond to the CTLA-4 sufficient or deficient mouse. Area under the curve (AUC) was also calculated for each mouse and the bar graph shows the mean ± SEM for each group. The maximum score for each mouse over the course of disease was identified to determine the severity (Max Score) with the bar graph showing the mean ± SEM for each group. Each experiment consisted of 4–8 mice, each experiment was repeated two separate times, and the mice from all experiments are pooled. Significance of EAU scores for each day was determined by two-way ANOVA with Bonferroni post-test. Significance of AUC and Max Scores was determined by nonparametric Mann-Whitney U test. Statistical significance (P ≤ 0.05) is designated by *, (P ≤ 0.01) is designated by **, and no significance is designated by “ns”.