Table 3 Primers used for quantitative real-time PCR in this study.
Genes | Accession no. | Sequence | Tm | Product size |
|---|---|---|---|---|
IL-1β | XM_005457887.1 | F: TGGTGACTCTCCTGGTCTGA R: GCACAACTTTATCGGCTTCCA | 60 | 200 |
GPX | NM_001279711 | F: GGTGGATGTGAATGGAAAGG R: CTTGTAAGGTTCCCCGTCAG | 60 | 190 |
β-actin | XM_003443127.5 | F: CCACACAGTGCCCATACTACGA R: CCACGCTCTGTCAGGATCTTCA | 54 | 112 |
TNF-α | NM_001279533 | F: CTTCCCATAGACTCTGAGTAGCG R: GAGGCCAACAAAATCATCATCCC | 60 | 161 |
NPY-α F | XM_003448854.5 | F: TCTCGCTCACTGCTGTCCC R: CAGAGGCGTGGTGTTCGTT | 60 | 72 |
Galanin | XM_003453581.5 | F: TGTTAGGGCCCCATGGACTA R: GAAGTCCTCCTCCTGGCCTA | 60 | 92 |
Ghrelin | AB104859.1 | F: GTGGTGCAAGTCAACCAGTG R: CATGGCTTGGCGACCAATTC | 59 | 97 |
Hsp70 | XM_019357557.1 | F: CGATGGCAGAAAAACTGACTCG R: GCTGCACTGAGGTCTAGCAG | 58.8 | 136 |
MHCII-α | AF212856.1 | F: ATCCACCATCAACGTTCCCT R: TCAGACCCACTCCACAGAAC | 58 | 68 |
Nrf2 | XM_003447296.4 | F: CTGCCGTAAACGCAAGATGG R: ATCCGTTGACTGCTGAAGGG | 57.5 | 69 |
EF-α | AB075952 | F: CTACAGCCAGGCTCGTTTCG R: CTTGTCACTGGTCTCCAGCA | 60 | 139 |
