Table 2 Precision and Reliability of the HIV-1 Tiling PCR Assay in key subgenomic regions.
Sample | Batch | Sample subtype | Post-dilution viral load (copies/mL) | Percentage pairwise identity in PR-RT (count > 98% identical) | Percentage pairwise identity in sequenced region (count > 98% identical) |
|---|---|---|---|---|---|
Intra-assay comparisons (Precision) | |||||
1 | 1 | B | 14,351 | 99.8 (10) | 99.8 (10) |
2 | 3 | CRF01_AE | 19,738 | 99.7 (10) | 99.8 (10) |
3 | 4 | B | 8296 | 99.2 (10) | 99.2 (10) |
Inter-assay comparisons (Reliability) | |||||
1 | n/a | B | 36,133 | 99.9 (10) | 99.8 (10) |
2 | n/a | C | 13,458 | 99.6 (10) | 99.7 (10) |
3 | n/a | B | 6646 | 99.9 (10) | 99.7 (10) |
