Table 1 Purification steps of chromate reductase from B. paramycoides S48.
From: Cloning and expression of chromate reductase from Bacillus paramycoides S48 for chromium remediation
Protein purification steps | Activity U/mL | Protein mg/mL | Specific activity U/mg | Yield (%) | Purification fold |
|---|---|---|---|---|---|
Crude extract | 432.9 | 2.02 | 214.0 | 100 | 1 |
(NH4)2SO4 precipitated | 797.3 | 1.54 | 516.7 | 92.0 | 2.4 |
Sephadax-G100 | 1171.5 | 0.82 | 1416 | 59.5 | 6.6 |
