Table 1 Primer sequence for the studied phylum and genus.
From: The gut dysbiosis of mothers with gestational diabetes and its correlation with diet
Target phylum/genus | Primer sequence (5 − 3) | Amplicon size (bp) | Annealing Temperature (ºc) | |
|---|---|---|---|---|
Firmicutes | F | GGAGYATGTGGTTTAATTCGAAGCA | 126 | 60 |
R | AGCTGACGACAACCATGCAC | |||
Bacteroidota | F | GGARCATGTGGTTTAATTCGATGAT | 126 | 60 |
R | AGCTGACGACAACCATGCAG | |||
Actinomycetota | F | TACGGCCGCAAGGCTA | 300 | 60 |
R | TARTCCCCACCTTCCTCCG | |||
Proteobacteria | F | CATGACGTTACCCGCAGAAGAA | 240 | 63 |
R | CTCTACGAGACTCAAGCTTGC | |||
Bifidobacterium spp. | F | TCGCGTCYGGTGTGAAAG | 243 | 60 |
R | CCACATCCAGCRTCCAC | |||
Bacteroides | F | GAGAGGAAGGTCCCCCAC | 106 | 60 |
R | CGCTACTTGGCTGGTTCAG | |||
E.coli | F | AGAAGCTTGCTCTTTGCTGA | 120 | 57 |
R | CTTTGGTCTTGCGACGTTAT | |||
Clostridium IV group | F | GCACAAGCAGTGGAGT | 230 | 60 |
R | CTTCCTCCGTTTTGTCAA | |||
16SrRNA | F | AAACTCAAAKGAATTGACGG | 180 | 60 |
R | CTCACRRCACGAGCTGAC | |||
