Publisher Correction: TSC complex decrease the expression of mTOR by regulated miR-199b-3p

Correction to: Scientific Reports https://doi.org/10.1038/s41598-025-85706-8, published online 13 January 2025

The original version of this Article contained errors in the order of the Figures and in the Funding section.

Figure 1 was published as Figure 5, Figure 2 was published as Figure 1, Figure 4 was published as Figure 6, Figure 5 was published as Figure 2, and Figure 6 was published as Figure 4. The Figure legends were correct.

The original Figures 1, 2, 4, 5 and 6 and accompanying legends appear below.

Fig. 1

Family pedigree and sequencing results. (A, E) Family pedigree, the families involved in this study. Squares indicate males, circles indicate females, the patients are shown as filled symbols. The proband is indicated by arrows(↗), the individual showed no genotype under the squares or circles indicate the genotype was not tested during this study. (B) The facial angiofibromas of the proband in family I. (C, F) Sanger sequencing analysis, the gene variation is shown by red arrows. (D, G) Evolutionary conservation of amino acid residues altered by the variations across different species.

Fig. 2

Expression of TSC2 and phenotypes of the fibroblasts with or without TSC2-Q371fs, the WT was present as the average of all healthy volunteers. (A) Linear schematic of the protein structure of TSC2-WT, TSC2-Q371fs and the location of the primes and the qRT-PCR results, relative expression levels were normalized with GAPDH. (B) Immunofluorescence analysis using TSC2 (red) antibody and DAPI (blue) in Bar 15 μm. (C) The western blot of the endogenous TSC2 and the statistics, the originals gels can be seen in the supplementary figure S1. (D) Proliferation rate of fibroblasts. (E) Apoptosis assay of fibroblasts and the statistics.

Fig. 4

miR-199b-3p regulate the expression of mTOR (A) Quantitative analysis of mTOR in fibroblasts using qRT-PCR. (B) Quantitative analysis of mTOR in fibroblasts using western blot and the statistics. The originals gels can be seen in the supplementary figure S5. (C) Predicted miR-199b-3p target sequence of the mTOR 3’UTR using miRBase. (D) Quantification of miR-199b-3p and mRNA using qRT-PCR, the WT represents the mixture of the equal amount total RNA from the two healthy fibroblasts. Relative expression levels were normalized with GAPDH. (E). Luciferase reporter assay. (F) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S5. (G) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with miR-199b-3p mimics,β-actin served as a control of total protein.(H) Quantitative analysis of mTOR in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p (low panel) and the statistics (up panel). (I) Western bolt analysis of p-AKT, AKT, p-p70S6K, p70S6K in HEK293T cells at 48 h post-treatment with the inhibitor specific to miR-199b-3p, β-actin served as a control of total protein. The originals gels can be seen in the supplementary figure S5.

Fig. 5

TSC complex regulates the endogenous content of miR199b-3p, *p < 0.05, **p < 0.01,***p < 0.001 comparison with WT. (A) The efficiency of knockdown TSC1 in shTSC1-HEK293T cell line. The originals gels can be seen in the supplementary figure S6. (B) The expression of miR-199b-3p in shTSC1-HEK293T cells and overexpression TSC1-WT or TSC1-N837fs in HEK293T cell line. (C) Quantitative analysis of mTOR in shTSC1-HEK293T cell line and overexpression TSC1-WT or TSC1 N837fs in HEK293T cell line using western blot (low panel) and the statistics (up panel). The originals gels can be seen in the supplementary figure S6. (D) The efficiency of knockdown TSC2 in shTSC2-HEK293T cell line. The originals gels can be seen in the supplementary figure S6. (E) The expression of miR-199b-3p in shTSC2-HEK293T cell line and overexpression TSC2-WT or TSC2- Q371fs in HEK293T cell line. (F) Quantitative analysis of mTOR in shTSC2-HEK293T cell line (low panel) and overexpression TSC2-WT or TSC2- Q371fs in HEK293T cell line using Western blot (low panel) and the statistics analysis (up panel). The originals gels can be seen in the supplementary figure S6.

Fig. 6

The molecular mechanism model of TSC complex regulate mTOR signal pathway through miR-199b-3p.

Finally, the Funding section was incorrect.

“This study was supported by the National Natural Science Foundation of China (No. 31700731, No.82070691) and the Grant of Natural Science Foundation of Shanxi Province in China (No.202203021212057).”

now reads:

“This study was supported by the National Natural Science Foundation of China (No.82070691) and the Grant of Natural Science Foundation of Shanxi Province in China(No.202203021212057).”

The original Article has been corrected.