Fig. 2

NALM6 cell invasion and proliferation after microinjection into cortical brain slices by fluorescence microscopy analysis. (A) Representative fluorescence images of cortical brain slices to longitudinally monitor NALM6-iRFP670 cells pretreated with Activin A (NALM6 Activin A) or not (NALM6 NS) up to 7 days post microinjection. Scale bar = 1 mm (B) Quantification of the area occupied by NALM6-iRFP670 up to 7 days after microinjection, as an index of brain invasiveness. (C) Quantification of NALM6-iRFP670 integrated density fluorescence signal up to 7 days after microinjection as an index of blast proliferation. Data are expressed as median + interquartile range (box) with whiskers showing min and max values. N ≥ 4 slices/group. Data are the results of 3 independent experiments. Data are analyzed by two-way ANOVA for repeated measurements, followed by Tukey’s multiple comparison test. Dotted line represents the baseline values obtained immediately after injection. (D) Overview image displaying NALM6-iRFP670 cell distribution in the cortical slice (dotted white line) 7 days after microinjection. The insert shows a representative 40 X magnification image with orthogonal projections showing NALM6-iRFP670 cells across the thickness of brain slice. Scale bar = 50 μm.