Fig. 3 | Scientific Reports

Fig. 3

From: Development of a 3D ex vivo model of brain-leukemia interaction to study the role of activin A in the central nervous system microenvironment

Fig. 3

NALM6 cell viability and proliferation after microinjection into cortical brain slices by flow cytometry analysis. (A) Flow cytometry plots showing Live/Dead cell staining in control (CTRL) slices, slices injected with NALM6-iRFP670 cells not stimulated (NALM6 NS) or pretreated with Activin A (NALM6 Activin A) at time 0, 24 h (h), 72 h and 7 days (d). Each plot was obtained from two pooled slices. (B) Percentage of viable NALM6-iRFP670 cells at time 0, 24 h, 72 h and 7 d after microinjection. (C) Graph showing the fold-change of NALM6-iRFP670 cells pretreated or not with Activin A at 0, 24 h, 72 h and 7 d after microinjection. The fold-change was calculated as number of total iRFP670-positive cells at the selected time-point/number of total iRFP670-positive cells at day 0. Data are represented as mean ± SEM, n = 2 replicate (each composed of 2 different slices).

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