Fig. 4 | Scientific Reports

Fig. 4

From: Development of a 3D ex vivo model of brain-leukemia interaction to study the role of activin A in the central nervous system microenvironment

Fig. 4

Activin A pretreatment of NALM6 cells induces higher cell death of brain tissue. (A) Representative images displaying propidium iodide (PI) signal in control slices (CTRL), and slices microinjected with NALM6 cells not stimulated (NALM6 NS) or pretreated with Activin A (NALM6 Activin A) at baseline (0, before injection), 24 h (h), 72 h and 7 days (d). Scale bar = 1 mm. (B) Fold-change representation of PI fluorescence signal at 24 h, 72 h and 7 d after leukemic cell microinjection over baseline (dotted line). Data are expressed as median + interquartile range (box) with whiskers showing min and max values. Data are obtained from 6 independent experiments (n > 20 slices/group). Data are analyzed by two-way ANOVA, followed by Tukey’s multiple comparison test.

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