Fig. 2 | Scientific Reports

Fig. 2

From: Strictosamide and mitraphylline inhibit cancer cell motility by suppressing epithelial-mesenchymal transition via integrin α4-mediated signaling

Fig. 2

The effects of strictosamide (1) and mitraphylline (2) on cell viability and motility of H1975, AGS, and CaCo2 cells. (A) The cell viability of H1975, AGS, and CaCo2 cells treated with strictosamide (1) and mitraphylline (2) for 48 h. Data are represented as the mean ± S.D. of the differences between the cell viability of cells affected with strictosamide (1) and mitraphylline (2) compared with that of DMSO-treated controls. (B) Representative images of each insert and relative number of invaded cells in the Transwell invasion assay. Cells were treated with strictosamide (1) and mitraphylline (2) at concentrations of 1 and 2.5 µM for 24 h. (C) Representative images of each wound area and quantitative data of relative wound density in the scratch wounding migration assay using the IncuCyte system. Overlaid on phase contrast images, the green line indicates the initial wound line, while the red line indicates the migrated cell area at the specified time period. Data are represented as the mean ± S.D. *p < 0.05; ** p < 0.01; *** p < 0.001.

Back to article page