Table 2 Primary and secondary antibodies used in Western blotting.
Name | Species | UniProt ID | Dilution | RRID number | Catalogue number | Supplier |
|---|---|---|---|---|---|---|
N-cadherin | Mouse | P19022 | 1:1000 | AB_398236 | 610921 | BD Bioscience |
FAK | Mouse | Q05397 | 1:1000 | AB_10610506 | sc-271195 | Santa Cruz Biotech |
p-FAK | Mouse | Q05397 | 1:1000 | AB_1125825 | sc-81493 | Santa Cruz Biotech |
β-actin | Rabbit | Q9UE89 | 1:1000 | AB_330288 | 4967 | Cell signaling |
Goat Anti-rabbit IgG (H + L) Peroxidase Conjugated | Goat | N/A | 1:10000 | AB_228341 | 31460 | Thermo Fisher Scientific |
Goat Anti-mouse IgG (H + L) Peroxidase Conjugated | Goat | N/A | 1:5000 | AB_228307 | 31430 | Thermo Fisher Scientific |
