Fig. 1

ALT001 alleviates paclitaxel-induced alterations in C4da neurons in Drosophila larvae. (A) Fluorescence images representing mt-Keima-labeled mitochondria in C4da sensory neurons of L3 control larvae (ppk^1a > mt-Keima) at abdominal segment A4 following 48 h treatment with DMSO (Veh) or ALT001 (750 µM) (left). Scale bars, 10 μm. Mitophagy levels in C4da sensory neurons were quantified across experimental groups (n = 12 per group) (right). (B) Representative images illustrating C4da neurons of L3 control larvae (ppk^1a > CD4-tdTom) at abdominal segment A4 following 48 h treatment with DMSO (Veh) or ALT001 (750 µM) (left). Enlarged views of the boxed regions are displayed in the lower panel. Scale bars, 50 μm. (C–D) Quantification of dendritic morphology, including dendrite length (C) and the number of dendritic branch points (D) in C4da neurons (n = 13–14 per sample). A single C4da neuron per larva was analyzed. Experiments were performed in triplicate, and data are presented as mean ± SD. Statistical significance was assessed using Student’s t-test (A) or one-way ANOVA followed by Sidák’s correction (C, D). Unless otherwise noted, differences are not statistically significant. ** p < 0.01; ***p < 0.001. NS, not significant.