Fig. 6
From: A tryptophol-containing emulgel ameliorates imiquimod-induced mice psoriasis
The TOH-containing emulgel alleviated activation of the JAK2/STAT3 pathway in inflammation and cell proliferation. (a) Immunohistochemical staining for JAK2, pJAK2, STAT3, and pSTAT3 on the skin section of all groups was performed. Representative images of skin sections were taken at ×40 magnification (scale bars: 50 μm). (b,c) Quantification of JAK2, and STAT3 positive cells in all groups (n = 4). (d,e) The mRNA expression of JAK2 and STAT3. (f) Protein expression of JAK2, pJAK2, STAT3 and pSTAT3 evaluated by western blot. (g,h) Relative protein expression levels of pJAK2 and pSTAT3 normalized to total JAK2 and STAT3, respectively (n = 4). (j,k) Quantification of pJAK2, and pSTAT3 positive cells in all groups (n = 4). (l,m) The mRNA expression of BCL2 and CCND1 were analyzed by RT-qPCR (n = 4). The data are presented as the means ± SD. Statistical significance was determined by one-way ANOVA. *p < 0.05, **p < 0.01, and ***p < 0.001.
