Fig. 4
From: SMARCB1 orchestrates cellular plasticity and oncogenic pathways in typical and chondroid chordomas
SMARCB1 knockdown promotes aggressive cellular behavior in chordoma cells. (A) Western blot analysis of SMARCB1 expression in wildtype, shNC, and shSMARCB1 chordoma cells. SMARCB1 protein levels are substantially reduced in shSMARCB1 cells compared to controls. (B) CCK8 proliferation assay comparing shNC and shSMARCB1 cells over 72 h. shSMARCB1 cells show significantly increased proliferation from 24h onwards. (C) IncuCyte real-time cell proliferation assay over 100 h. shSMARCB1 cells demonstrate markedly accelerated growth compared to shNC controls. (D) Flow cytometry analysis of cell cycle distribution. Left: Representative DNA content histograms. Right: Quantification of cell cycle phases. shSMARCB1 cells exhibit an increased G0/G1 population and decreased G2/M population relative to shNC. (E) Transwell migration assay. Left: Representative images of migrated cells stained with crystal violet. Right: Quantification of migrated cell numbers. shSMARCB1 cells show significantly enhanced migratory capacity. (F) Transwell invasion assay. Left: Representative images of invaded cells stained with crystal violet. Right: Quantification of invaded cell numbers. shSMARCB1 cells display markedly increased invasive potential. (G) Wound healing assay. Left: Representative phase-contrast images of wound closure at 0-, 24-, 48-, and 72-h post-scratch. Right: Quantification of wound area over time. shSMARCB1 cells exhibit wound closure at a rate comparable to shNC controls. (B–G) Data are presented as mean ± s.d. n.s., not significant; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (unpaired two-tailed Student’s t-test).
