Table 2 List of specific primers, PCR protocols, and PCR product sizes for three medicinal leeches.
Gene | Primer name | Primer sequences | PCR protocols (35 cycles) | Production size |
|---|---|---|---|---|
ND1 | ND1F1 | CCTATGGCTGATGCHA | Denaturation at 95°C for 5min, annealing at 95°C for 45 s, extension at 48°C for 1min, and a final extension at 72°C for 10 min | 251 bp |
ND1R1 | CCTAAYAAAGCATATTTTGAATT | |||
16S rDNA | 16SF1 | CTGACTGTGCAAAGGTAGCA | 212 bp | |
16SR1 | AGATCGCCCCAATCAAAAT | |||
12S rDNA | 12SF1 | ATYYGATAGTCCACGHT | 158 bp | |
12SR1 | TAAATAAATGTARCACAY | |||
COX1 | COX1F1 | TATTATGTAGTAGCTCATT | Denaturation at 95°C for 5min, annealing at 95°C for 45 s, extension at 45.5°C for 1min, and a final extension at 72°C for 10 min | 210 bp |
COX1R1 | TATCGYCGWGGTATTC |
