Fig. 2

Screening for anti-HIF1a auto-antibodies by Western blot. (a) As control, reactivity of healthy patient sera, patients with Type 1 diabetes sera and anti-HIF1a monoclonal antibody to recombinant HIF1a. (b) Reactivity of the patient to recombinant HIF1a. Band intensity was quantified and is represented in the adjacent graph. c. Reactivity of the patients’sera before and after immunodepletion to recombinant HIF1a.