Fig. 6

The dynamin disruptor Dyngo4a modulates uptake and subcellular localization of RGD-C’Dots in osteocytes in vivo. A-D) Short incubation experiments highlight changes between non-treated and Dyngo4a treated groups over time via linear regressions of cell count and t-tests of subcellular localization. Slopes of the linear regressions were compared between groups (differences indicated by * symbols). If the slopes were not significantly different, y-intercept comparisons were also made between groups (differences indicated by # symbols). E-H) In the long incubation experiment, dynamin disruption causes males to have a large increase in C’Dot uptake and to retain signal for over an hour longer compared to controls (2-Way ANOVA). ANOVA comparisons are shown between long incubation groups across all timepoints. Males also display a decrease in discrete subcellular localization in the long incubation experiment (t-test). Females show no differences in uptake, retention, or subcellular localization compared to controls. I) Receptor–mediated endocytosis is a sensitive uptake pathway that occurs after a receptor has been bound and is disrupted with application of Dyngo4a. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001; Error bars indicate SEM).