Fig. 5
Schematic overview of the study design for identifying potential osteoporosis therapeutic targets and compounds. Integrated bioinformatics analysis of human single-cell and murine bulk RNA sequencing datasets was employed to identify osteoporosis-associated genes, highlighting chondrogenesis-related genes such as CHAD and COL2A1. These targets were used for screening a Traditional Chinese Medicine (TCM) database, followed by molecular docking to prioritize candidate compounds. Subsequent in vitro validation of a top candidate, wogonin, demonstrated its effects on chondrogenic differentiation, suggesting its potential for further investigation.
