Fig. 3
From: SRT2104 enhances dendritic outgrowth and spine formation through Sirtuin 1-mediated mTORC1 signaling
Effects of SRT2104 on the levels of mTORC1, 4E-BP1 and p70S6K phosphorylation in dexamethasone-treated cells. Cortical cells were treated with SRT2104 (0.1 and 1 µM) or DMSO for 4 days in the presence of dexamethasone. The levels of phospho-Ser2448-mTORC1 (A), phospho-Thr37/46–4E-BP1 (B) and phospho-Thr389-p70S6K (C) were determined by Western blotting (n = 6–8). Full-length uncropped gels for all Western blots are available in the Supplementary Information. The values represent the mean ± SEM expressed as a percentage of control cells (DMSO-treated, non-dexamethasone-treated cells). *P < 0.05 or ***P < 0.001 vs. control cells; †P < 0.05, ††P < 0.01 or †††P < 0.001 vs. DMSO-treated, dexamethasone-treated cells.
