Fig. 6
From: SRT2104 enhances dendritic outgrowth and spine formation through Sirtuin 1-mediated mTORC1 signaling
Effects of sirtuin 1 knockdown on the levels of ERK1/2 and mTORC1 phosphorylation in primary cortical cells. Cortical cells were transfected with fluorescein-labeled control or sirtuin 1 siRNA (100 nM) for 24 h. The levels of sirtuin 1 (A), phospho-Thr202/Tyr204-ERK1/2 (B) and phospho-Ser2448-mTORC1 (C) were determined by Western blotting (n = 6). Full-length uncropped gels for all Western blots are available in the Supplementary Information. The values represent the mean ± SEM expressed as a percentage of control cells (control siRNA-transfected cells). ***P < 0.001 vs. control cells.
