Fig. 7

Effects of sirtuin 1 knockdown on dendritic outgrowth and spine density in primary cortical cells. Cortical cells were transfected with fluorescein-labeled control or sirtuin 1 siRNA (green) for 24 h. In total, 600 cells from each group were analyzed using anti-MAP2 (red) to assess neurite length (A-a), neurite number (A-b), branch points (A-c), and soma area (A-d). In total, 45 dendritic segments from each group were analyzed using phalloidin dye (red) for spine formation (B). All data are expressed as the mean ± SEM. All data are expressed as the mean ± SEM. ^*P < 0.05, ^**P < 0.01 or ^***P < 0.001 vs. control cells (control siRNA-dtransfected cells).