Fig. 1
Characteristics of human placenta-derived mesenchymal stem cells (hP-MSCs). (A) Morphology and differentiation potential of hP-MSCs. (a, b) The morphology of hP-MSCs cultured in complete medium on day 2 and day 14. (c, d) hP-MSCs cultured in osteogenic differentiation medium after Alizarin Red S staining compared to the control group cultured in DMEM + 10% FBS. (e, f) hP-MSCs cultured in adipogenic differentiation medium after Oil Red O staining compared to the control group cultured in DMEM + 10% FBS. (g, h) hP-MSCs aggregate cultured in a chondrogenic differentiation medium after Alcian blue staining compared to the control group cultured in DMEM + 10% FBS. (B) Flow cytometry analysis showed the expression of typical MSC surface markers, CD73, CD90, CD105, and the absence of hematopoietic markers, CD34, CD45, and HLA-DR. Images (a, b, g, and h) were captured with a 10 × magnification. Scale bar = 200 µm; Images (c and d) were captured with 4 × magnification. Scale bar = 500 µm; Image e was captured with 20 × magnification. Scale bar = 100 µm; Image (f) was captured with 40 × magnification. Scale bar = 50 µm.
