Table 1 List of primers used for qRT-PCR.
From: Chemotherapy treatment alters DNA methylation patterns in the prefrontal cortex of female rat brain
Target gene | Primer Sequence (5’-3’) |
|---|---|
DNMT1 | (+) TACGCAAGGTTTGAGTCCCC (–) CCCAGTCGGTAGACAACACC |
DNMT3a | (+) GTTCAACCTCCCACCTCTTCA (–) TGCCTGGGGTGTCCAAAAA |
MeCP2 | (+) ATGGTAGCTGGGATGTTAGGG3 (–) TGAGCTTTCTGATGTTTCTGCTT |
CBP | (+) AAGCAACAAATTGGGTCACCAGGC (–) TACTAAGGGATGTGGCGATCTGCT |
CREB | (+) CGTCATCTGCTCCCACTGTA (–) CCTTCGTTTTTGGGAATCAG3 |
TET1 | (+) GAGCCTGTTCCTCGATGTGG (-) CAAACCCACCTGAGGCTGTT |
Wnt3 | (+) GCCTCTGACAAGCCCGAAA (-) GCGACGCCCCCAATAGTT |
CNP | (+) CACCATGCACCTCTCCCAGC (-) ATGGAGCCGATCCGGTCCAG |
MAG | (+) GCCAGACCATCCAACCTTCTGT (-) AAGTCGAAACGGCAGGGGAT |
18 S rRNA | (+) CATTCGAACGTCTGCCCTAT (–) GTTTCTCAGGCTCCCTCTCC |
