Fig. 3 | Scientific Reports

Fig. 3

From: Valproate independently activates Snf1, inhibits TORC1, and induces repression of INO1 transcription by increasing nuclear localization of Opi1

Fig. 3

VPA-induced repression of INO1 is mediated by the transcriptional repressor Opi1. (A) WT and opi1∆ were inoculated in inositol-free medium (I-) and incubated until the log growth phase when VPA and/or inositol (I + ; 75 µM) were administered for 2 h. INO1 mRNA was assayed by RT-qPCR analyses. n = 3 biological replicates; error bars are SEM; two-way ANOVA * = p < 0.05, **** = p < 0.0001 compared to WT control I-. (B) A strain endogenously expressing OPI1-mGFP was cultivated to the mid-log growth phase and split into three parts. Each portion was treated with either inositol (75 µM), VPA (1 mM), or vehicle (control). Live cells were then stained with DAPI (5 µg/mL) for 30 min prior to continuously monitoring Opi1-mGFP localization by confocal microscopy imaging up to 2 h after treatment additions. Scale bar in top left most figure represents 2.5 µm.

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