Fig. 4 | Scientific Reports

Fig. 4

From: Valproate independently activates Snf1, inhibits TORC1, and induces repression of INO1 transcription by increasing nuclear localization of Opi1

Fig. 4

VPA and rapamycin do not repress INO1 mRNA and MIPS expression during the diauxic shift/stationary phase. (A) Experimental diagram of yeast culture growth before and after treatments. Yeast cells were inoculated in inositol-free medium and incubated until the late-log growth phase when treatments of VPA (1 mM), rapamycin (400 nM), or vehicles (control) were administered. Samples were collected over a 24 h period. (B) INO1 mRNA expression levels, (C) MIPS protein levels, and (D) PP-Rps6 levels (a TORC1 activity biomarker) were assayed by RT-qPCR and SDS-PAGE/WB analyses. mRNA data is shown relative to 24 h control. WB data is shown relative to 0 h control. Total protein normalization (TPN) was used to normalize WB quantifications. n = 2 biological replicates; error bars are SEM.

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