Fig. 5
Expression of senescence markers and SASP components in HCC patients. (a) Representative Western blot images, and (b) quantitative analysis of NF-κB, p53, p21, and p16 expression in tumor (T) and adjacent non-tumor (A) tissues. (c) RT-qPCR analysis of p53, p21, p16, and SASP component (IL-1β, IL-6, IL-8, TNF-α) levels in tumor (T) and adjacent non-tumor (A) tissues. (d) Correlation analysis of NFATc1 levels with cell cycle markers (p53, p21, p16) and SASP components (NF-κB, IL-1β, IL-6, IL-8, TNF-α) in tumor samples from HCC patients. Statistical analysis: Student’s t-test for two-group comparisons, Spearman correlation for the associations between NFATc1 and cell cycle markers or SASP components. Benjamini-Hochberg FDR correction (q < 0.1) was applied to the correlation p-values. n = 12, **p < 0.01, ***p < 0.001, ****p < 0.0001.
