Fig. 3

Western blot analysis of the effects of compounds 15a and 15d on the acetylated histone levels in HepG2 cells. (A) HepG2 cells were treated with 15a, 15d, and SAHA for 24 h at the indicated concentrations. The acetylated H3/H4 protein was determined by Western blot analysis, and β-actin was detected as the endogenous loading control, accordingly. (B) Quantitative analysis of (A). The data are expressed as means ± SD of three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus control.