Fig. 5

Scanning electron micrographs illustrating the midgut morphology of G. mellonella obtained from control larvae (A-C) and CdO NP-treated group (A`- C`). (A) illustrate the lumen of midgut of the control group. (B) illustrates the typical cytoplasmic protrusions in between the midgut enterocytes. (C) exposes cell boundaries, microvilli, and peritrophic matrix. (C*) presents higher magnification of panel B. (A`) illustrates morphologically altered lumen of midgut of the treated group. (B`) shows rupture of plasma membranes of cytoplasmic protrusions (purple arrow). (B*) Privileged enlargement of the inset in A`. (C`) depicts the altered morphology of midgut lumen. (Blue circle) presents the damage of the peritrophic matrix. (A and A`) (scale bar = 50 µm and magnification X 500), (B and B`) (scale bar = 5 µm and magnification X 3000), (B*) (scale bar = 1 µm and magnification X 14,000),C and C` (scale bar = 5 µm and magnification X 5000), (C*) (scale bar = 1 µm and magnification X 10,000).