Fig. 5
From: Dbl2 interacts with helicases and an endonuclease to maintain the integrity of repetitive regions
Dbl2 and Fbh1 prevent chromosome III instability and deletions. (A, B) PFGE analyzes of chromosomes from pat1-114 control cells (SP3), pat1-114 dbl2Δ (SP511), and pat1-114 fbh1Δ mutant cells (SP567). Equal numbers of cells were prepared in agarose gel plugs from meiotic and stationary phase cultures in triplicates. Synchronous meiosis was induced by Pat1 inactivation in cells arrested at the G1 phase, and samples of cells were taken 5.5 h after the induction of meiosis. Stationary phase cultures were prepared by 2-week cultivation on YES plates. (C) Analysis of the length of rDNA arrays by Southern hybridization. Chromosomes in plugs from (A and B) were digested by ScaI restriction enzyme, separated by PFGE, and hybridized with [α32P] ATP-labelled rDNA probe. The position of rDNA fragments after Southern hybridization is marked with red arrows.
