Fig. 6

Effect of deleting fbh1, rad51, and rad52 in wild-type and dbl2Δ strains on Tf2 stability. (A) Two possible ways of ura4 loss, gene conversion, and deletion by LTR recombination, are depicted. (B) 12 independent colonies of wild-type (SP1360) and deletion strains (SP101, SP92, SP305, SP333, SP1423, SP87, SP290, SP1885) were grown in YES at 32 °C to saturation and plated onto YES + 5-FOA selective plates. Growing colonies were counted and characterized for the rearrangements by colony PCR with primers Tf2_6-F + Tf2_6R (deletion) and Tf2_6F + Tf2_2R (conversion). Statistical significance of any differences between the wild-type and mutant strains or between single and double mutants was assessed by Student’s T-test and is depicted as *p < 0.05, **p < 0.01, ***p < 0.001.