Fig. 1 | Scientific Reports

Fig. 1

From: Novel single-cell preservation and RNA sequencing technology unlocks field studies for Plasmodium natural infections

Fig. 1

Experimental setup to test methods for processing Plasmodium natural infections and in vitro cultures. A mock natural infection sample was prepared by mixing P. knowlesi A1-H.1 in vitro culture with human whole blood. Two combinations of leukodepletion and parasite enrichment methods were tested (PNyco and MACSPS). In addition, we processed an in vitro-cultured P. knowlesi sample, with two different density gradients: Nycodenz (PkCultureN) and Percoll (PkCultureP). For each sample 60,000 cells were loaded into a HIVE by gravity (G) or centrifuge (C). Figure created using BioRender.com.

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