Fig. 5

Microscopy and x-ray imaging of BBD SN1 and DCIS SEN calcifications associated with BBD and DCIS. (a) BF microscopy image of a histology section from BBD biopsy MCT006 exhibiting cysts and ACP SN. (b - d) 3D surface rendered Micro-CT scan lateral view of the paraffin embedded block from which the histology section in a was prepared. Volume rendered raw Micro-CT data (b), 3D surface-rendered Micro-CT data with color coded sphericity analyses (c) and an overlay of image b on image c (d). (e and f) Micro-CT 3D surface rendered image enlargements of c with sphericity analyses. (g) BF microscopy image of a histology section from DCIS biopsy MCT009 exhibiting ACP SEN within ducts. (h - j) 3D surface-rendered Micro-CT scan lateral view of the paraffin embedded block from which the histology section in g was prepared. Volume rendered raw Micro-CT data (h), 3D surface rendered Micro-CT data with sphericity analyses (i) and an overlay of image h on image i (j). (k and l) Micro-CT 3D surface-rendered image enlargements of i with sphericity analyses. While variable, the largest ACP SN associated with BBD are significantly smaller than the ACP SN and ACP SEN deposits in DCIS. Micro-CT = micro-computed tomography. ACP = amorphous calcium phosphate and amorphous calcium phosphate transforming toward HAP. HAP = hydroxyapatite. SN = spheroidal nodules. SEN = semi-ellipsoidal nodules. BBD = benign breast disease. DCIS = ductal carcinoma in situ. HS = histology section. 3D = three-dimensional.