Fig. 6
Microscopy and spectroscopy of SN1 calcifications, cholesterol and waxy substances (WS) associated with BBD. (a - h) Paired TPMT, CPOL, SRAF and SRIF imaging of BBD biopsy MCT006, exhibiting highly birefringent WS and euhedral cholesterol precipitated on the inner walls of BBD acini. (i) A representative 300–3500 cm− 1 Raman spectrum from Raman spot analyses (n = 50) within BBD (a - h), with expanded spectral regions for WS at 800–1500 and 2700–3000 cm− 1. See experimental section for details on how peak fitting analyses were performed63,64,65,66,67,68. Euhedral morphology (g, h) indicates cholesterol composition, which has been confirmed with Raman analyses in previous studies44. ACP = amorphous calcium phosphate and amorphous calcium phosphate transforming toward hydroxyapatite. SN = spheroidal nodules. BBD = benign breast disease. TPMT = transmitted light photomultiplier tube. CPOL = circular polarization. SRAF = super resolution autofluorescence. SRIF = super resolution induced fluorescence.WS = waxy substances. HS = histology section. OPN = osteopontin.
