Fig. 8 | Scientific Reports

Fig. 8

From: Mechanisms of osteopontin-stabilized amorphous calcium phosphate calcification in benign and pre-malignant breast disease

Fig. 8

Microscopy of SN3 calcifications. Spatially correlated TPMT, CPOL, SRAF and SRIF imaging of SN3 calcifications in DCIS biopsies MCT002 (a and i) and MCT009 (j and l), which indicate that SN3 calcifications formed in contact with necrotic cells (a – l). White boxes in a, b and c indicate areas of SN3 enlargements in d – f and g – i, respectively. OPN forms coatings and is entombed within SN3 deposits (c, f, i, l). SN3 calcifications were crushed, fractured and partially removed during sectioning (g – i). ACP transformation toward HAP is exhibited by euhedral forms (f, g – i). TPMT = transmitted light photomultiplier tube. CPOL = circular polarization. SRAF = super resolution autofluorescence. SRIF = super resolution induced fluorescence. ACP = amorphous calcium phosphate and amorphous calcium phosphate transforming toward hydroxyapatite. SN = spheroidal nodules. HAP = hydroxyapatite. BBD = benign breast disease. BF = bright field. HS = histology section. WS = waxy substances. OPN = osteopontin.

Back to article page