Fig. 4

The BAI1-expressing cell population in the alveolar lumen consisted mainly of AMs (A, B). Cells in BALF were separated from each group of mice for immunofluorescence staining, and the expression of BAI1 was visualized using laser confocal microscopy. Blue fluorescence corresponds to DAPI-stained nuclei; Red fluorescence represents CD11c stained with anti-CD11c antibody, marking AMs; Green fluorescence represents BAI1 stained with antibody PA5-75348. Merge: Overlap of three fluorescence spectra at the same position. Scale bars: A, B = 50 μm.